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Title: Development of ISSR derived SCAR marker for economically important Benstonea thwaitesii (Martelli) Callm. and Buerki
Authors: Borse, Vrushali
Zanan, Rahul
Nadaf, Altafhusain
Keywords: Benstonea thwaitesii;Pandanaceae;ISSR;SCAR
Issue Date: Jul-2018
Publisher: NISCAIR-CSIR, India
Abstract: Benstonea thawaitesii (Martelli) Callm. and Buerki, an economically important plant of Pandanaceae family, is having fragrant male inflorescence. In vegetative growth phase, it is difficult to distinguish B. thwaitesii from other Pandanaceae species due to similar morphological characters. In the present study, we have developed inter simple sequence repeat (ISSR) derived sequence characterized amplified region (SCAR) marker for identification of B. thwaitesii. A set of 15 Indian Pandanaceae species was screened using 15 ISSR primers. Among 15 primers, UBC-857 generated unique 1 kb band specific to B. thwaitesii. It was further cloned, sequenced and used to design SCAR primer that amplified the expected amplicon of 750 bp in B. thwaitesii only. This is the first report of developing species-specific SCAR marker in B. thwaitesii using ISSR primers.
Page(s): 480-483
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(3) [July 2018]

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