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|Title:||DNA fingerprinting of peach (Prunus persica) germplasm in accessing genetic variation using arbitrary oligonucleotide markers system|
|Keywords:||Genetic diversity;Peach;Prunus persica;RAPD;ISSR;Molecular markers|
|Abstract:||Molecular characterization of 45 peach (Prunus persica) accessions was carried out using 48 RAPD and 46 ISSR molecular markers to assess the value and magnitude of genetic divergence. The RAPD primers revealed 84.20% polymorphism and ISSR markers generated 89.00% polymorphism. Pooled RAPD and ISSR along with UPGMA clustering based on Jaccard’s coefficient were estimated with a view to assess efficiency of the marker system in Prunus persica. Polymorphic information conten (PIC) values varied from 0.13 to 0.50 in RAPD and 0.12 to 0.49 in ISSR with the mean values for all loci were 0.33 and 0.35, respectively. Jaccard's similarity coefficient among peach accessions with respect to RAPD and ISSR markers ranged from 0.37 to 0.95 and 0.43 to 0.95 which indicated a broad genetic base. Pooled analysis of both molecular markers concluded that genotypes ‘Darli’ and ‘IC-2’ are most distantly related to each other. The use of arbitrary oligonucleotide primers in the amplification reaction facilitated the study of uncharacterized genomes. In the present study, high level of polymorphism indicates their applicability in framing more extensive studies in development of superior progenies, quantitative trait loci (QTL) mapping, molecular breeding, investigation of population genetic diversity, comparative mapping, selection of the parents etc. among various peach crop improvement programmes.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.17(3) [July 2018]|
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