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Title: Assessment of genetic fidelity of long term micropropagated shoot cultures of Achras sapota L. var. ‘Cricket Ball’ as assessed by RAPD and ISSR markers
Authors: Chittora, M.
Keywords: Achras sapota;Genetic fidelity;RAPD;ISSR
Issue Date: Jul-2018
Publisher: NISCAIR-CSIR, India
Abstract: RAPD and ISSR molecular markers were employed to evaluate genetic fidelity of shoots of Achras sapota L. var. ‘Cricket Ball’ raised through nodal bud cultures. Out of 53 RAPD and 60 ISSR primers screened, 34 RAPD and 20 ISSR primers generated a total of 270 and 120 clear and scorable amplification products, respectively. All the shoot cultures analyzed at different culture passages showed similar RAPD and ISSR profiles as compared to that of mother plant. No genetic variation was observed in in vitro shoot cultures. The results indicated the genetic stability of the tissue culture raised shoot cultures of A. sapota and corroborate the assumption that axillary multiplication is the safest mode for multiplication of true to type plants without any somaclonal variation.
Page(s): 492-495
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(3) [July 2018]

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