Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/45275
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSingh, Anita-
dc.contributor.authorSharma, J K-
dc.date.accessioned2018-10-23T12:05:48Z-
dc.date.available2018-10-23T12:05:48Z-
dc.date.issued2018-07-
dc.identifier.issn0975-0967 (Online); 0972-5849 (Print)-
dc.identifier.urihttp://nopr.niscair.res.in/handle/123456789/45275-
dc.description496-503en_US
dc.description.abstractMaize is an important cereal crop of North-Western Himalayan region of India with wide ranging and diversified uses which is resulting in the release and recommendation of new hybrids of maize for the region. The use of parental lines with a narrow genetic base by various private and public sector seed agencies may cause a risk of genetic diversity loss. Molecular characterization of hybrids and parental lines by DNA fingerprinting provides knowledge of the genetic relationship among them thus preventing the risk of increasing uniformity. The technique is helpful for molecular identification and hybrid purity testing. The present study was undertaken to carry out microsatellite marker based DNA fingerprinting of ten maize hybrids and their parental lines for molecular identification and assessment of genetic diversity. Twenty three microsatellite markers were found to be polymorphic. Number of alleles per locus ranged from 2 to 6 with a mean of 3.30. Mean expected heterozygosity and polymorphic information content was 0.58 and 0.52, respectively. Average Jaccard’s similarity coefficient observed was 0.46 revealing an average genetic diversity of 54% among various genotypes studied. Cluster analysis delineated the maize hybrids and parental lines into four main clusters A, B, C and D at 0.32 similarity coefficient.en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBT Vol.17(3) [July 2018]en_US
dc.subjectMicrosatellite markersen_US
dc.subjectMaizeen_US
dc.subjectMolecular characterizationen_US
dc.subjectExpected heterozygosityen_US
dc.subjectPolymorphic information contenten_US
dc.titleMolecular characterization of parental lines and hybrids of maize cultivated in North-Western Himalayan region using microsatellite markersen_US
dc.typeArticleen_US
Appears in Collections:IJBT Vol.17(3) [July 2018]

Files in This Item:
File Description SizeFormat 
IJBT 17(3) 496-503.pdf345.73 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.