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|Title:||Usefulness of three DNA-PCR techniques to differentiate Jalapeño pepper varieties|
Teodoro-Pardo, Claudia Verónica De
|Keywords:||DNA fingerprints;Capsicum annuum;Jalapeno variety;Molecular discrimination;Electrophoresis;Mexico|
|Abstract:||The cultivation of pepper (Capsicum annuum) is one of the oldest in America. However, out of the 25 species described only 5 have been domesticated. The varieties registered and legally protected must comply with International Union for the Protection of New Varieties of Plants (UPOV) guidelines. Nevertheless, simple sequence repeat the morphological descriptors used for this purpose are sometimes subjective and they are altered by the environment conditions. Molecular genetic markers have been used as alternative to reduce these limitations. The aim of this study was to compare the effectiveness of three techniques based on PCR to discriminate varieties of jalapeño pepper with little phenotypic differences and with very similar shape of the fruit. The techniques used were random amplified polymorphic DNA-DNA amplification fingerprinting (RAPD-DAF), inter simple sequence repeat (ISSR) and simple sequen repeat (SSR) through capillary and conventional electrophoresis. Results indicated that the values of genetic variation in the studied jalapeno varieties are different depending on the technique used, which is due to the capacity of each technique to sample the genome.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.17(3) [July 2018]|
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