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Title: Isolation and amplification of genomic DNA from nutmeg mace
Authors: Swetha, V P
Parvathy, V A
Sheeja, T E
Sasikumar, B
Keywords: Nutmeg;Myristica fragrans;Recalcitrant;Restriction digestion;RAPD;rbcL;ITS
Issue Date: Jul-2018
Publisher: NISCAIR-CSIR, India
Abstract: A reliable and efficient protocol for isolation and amplification of genomic DNA from dried mace of Myristica fragrans, was developed. The yield of genomic DNA was 231.4 µg g-1 and 306.8 µg g-1, respectively for the samples procured from the farm and market. The absorbance ratio at A260/A280 was greater than 1.8 indicating the good quality of DNA. Complete restriction digestion and PCR amplification of genomic DNA further confirmed the quality of isolated DNA.
Page(s): 533-537
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(3) [July 2018]

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