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Title: Identification and Characterization of a Protease Encoding Metagenome Clone from Dairy Effluent
Authors: Lal, R
Soni, K B
Alex, S
Sreekantan, L
Reghunath, B R
Keywords: Metagenome;DNA;Transformed
Issue Date: Oct-2018
Publisher: NISCAIR-CSIR, India
Abstract: Metagenomic DNA was isolated from dairy effluent collected from a milk processing unit. The Hpa I digests of metagenome were cloned in pEZ BAC vector. The transformed DH5 colonies were selected by blue white screening on chloramphenicol (25μg/ml) containing plates. Functional screening of the library on skim milk agar showed a clone having protease activity. This clone was confirmed to have a metagenome insert of size approx. 9kb. The crude cell extract of this clone at 24th h of growth showed protease activity of 166.99U/ml (111.32U/mg protein). The protease was found to be alkaline and had maximum activity at 400C and pH 9.0.
Page(s): 583-586
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.77(10) [October 2018]

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