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Title: A two locus barcode for discriminating Piper nigrum from its related adulterant species
Authors: Parvathy, V A
Swetha, V P
Sheeja, T E
Sasikumar, B
Keywords: DNA barcoding;psbA-trnH;rpoC1;rbcL. Piper nigrum;Single locus
Issue Date: Apr-2018
Publisher: NISCAIR-CSIR, India
Abstract: DNA barcoding in plants involves the selection and sequencing of DNA regions as a tool for species identification. Here we have attempted to discriminate Piper nigrum L., black pepper of commerce, from its related adulterant species viz., Piper attenuatum Buch-Ham, Piper galeatum (Miq.) C. DC. using rbcL, psbA-trnH, rpo'C'l, and matK as barcode loci. No single barcoding locus could discriminate all the three species of Piper. However rbcL and rpoC1 could differentiate P. attenuatum from P. nigrum and Piper galeatum while psbA-trnH differentiated P. galeatum from P. nigrum and P. attenuatum. Two locus barcode approach proved better when compared to single locus in discriminating Piper species. The coding rbcL gene with non coding psbA-trnH spacer was preferred than the coding rpoC1 + psbA-trnH combination in the present study.
Page(s): 346-350
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(2) [April 2018]

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