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Title: Fruit size homologue gene variation in Mexican Opuntia sp.
Authors: Samah, Samir
Valadez-Moctezuma, Ernestina
Keywords: Nopal;Opuntia sp.;Genetic variation;Fruit size homologue gene
Issue Date: Apr-2018
Publisher: NISCAIR-CSIR, India
Abstract: The Opuntia genus, an important horticultural crop in Mexico, is essentially a fruit crop. The fruits (“tunas” and “xoconostles”) exhibit a huge range of color and size but the genetic background of this diversity is little known. The aim of this research was to amplify a fruit size gene and to estimate the changes in the gene sequence in 91 genotypes of Mexican Opuntia. To accomplish this, the cleaved amplified polymorphic sequences (CAPS) technique was applied. A fragment of 850 bp was obtained using the primer pair Fruitfull-F131/Fruitfull-R1218 in all Opuntia genotypes studied here. There was no length variation observed among the undigested PCR products from the 91 accessions. The amplified PCR products (FRUITFULL-homologue gene) were digested with seven endonuclease enzymes. The enzymes HinfI and RsaI had no restriction site, while the enzymes AvaII and HhaI generated two monomorphic bands. However, the enzymes AluI, HaeIII and MboI produced several polymorphic fragments. The estimated Dice similarity coefficient ranged between 0.46 and 1.0. However, the most frequent estimated Dice similarity coefficient varied from 0.75 to 1.0. Indeed, 63 out of the 91 genotypes had the same genetic profile. The present results revealed that the FRUITFULL-homologue gene is conserved among Opuntia genotypes.
Page(s): 351-356
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(2) [April 2018]

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