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|Title:||Plant regeneration from leaf explants in apple rootstock MM111: Effect of cytokinins and auxins|
|Keywords:||Adventitious shoot regeneration;Apple rootstocks;Malling-Merton111;Thidiazuran|
|Abstract:||A reproducible protocol for adventitious shoot formation has been achieved in commercially important clonal apple rootstock Malling-Merton111 (MM111). The procedure consists of inducing leaves excised from micropropagated shoots, on Murashige and Skoog (MS) medium supplied with various concentrations and combinations of cytokinins benzyl adenine (BA), thidiazuran (TDZ) and auxins indole butyric acid (IBA), naphthalene acetic acid (NAA), indole acetic acid (IAA), and 2-4, diphenoxy acetic acid (2,4-D). Frequency of regeneration was influenced by the concentration and type of cytokinin and auxin, light and dark incubation and length of explant exposure to plant growth regulators. Shoots were regenerated either through callus or directly from leaves on regeneration medium incubated in light/dark. However, number of explants regenerated and number of shoots were highest in explants incubated in light. Among BA treatments, optimum shoot regeneration (34%) was obtained at 3 mg l-1 BA with 1 mg l-1 NAA, while TDZ treatments (0.6 mg l-1 TDZ with 0.5 mg l-1 NAA) resulted in higher regeneration rate (44%), and produced increased number with longer shoots. The potential of regeneration was further increased at 0.6 mg l-1 TDZ with 0.5 mg l-1 NAA by using a two stage procedure where leaves were incubated on regeneration medium for 10 days and then transferred to basal medium. Here, 49% shoots originated directly with maximum of six shoots per regenerating explant in comparatively less time period while BA supplemented medium did not promote shoot induction in this experiment. Regenerated shoots were rooted successfully and the resulting plantlets were hardened in the potting mixture.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.17(2) [April 2018]|
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