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|Title:||Molecular characterization of new oat crown rust resistant sources|
|Keywords:||Crown rust resistance;Oat differential lines;RAPD|
|Abstract:||Crown rust, caused by the fungus Puccinia coronata f. sp. avenae, is a widespread and damaging disease of oat. Spontaneous oat species are an important and abundant source of new rust resistance genes. Two Tunisian resistant spontaneous oat accession JT0 and JT5 (Avena sterilis) was characterized using 21 RAPD primers and compared to the differential lines Pc38, Pc39 and Pc68 (Avena sativa L.), which showed previously a high resistance level to the local population of crown rust. The effectiveness of molecular markers could be evaluated through such parameters as the polymorphic information content (PIC), marker index (MI), heterozygosity (H) and the resolving power (Rp). Banding patterns obtained by RAPD analysis revealed polymorphism between accessions & RAPD profiles showed 33 common bands, 4 markers specific to A. sterilis accessions and 3 markers specific to A. sativa species. JT0 showed the lowest RAPDs number (61) among oat accessions. The Neighbor-Joining dendrogram inferred from the Slatkin’s pair wise distances (FST) showed that JT0 accession was clearly separated from the other accessions. JT5, Pc68, Pc38 and Pc39 were clustered in one group. It’s interesting to state that an A. sterilis line (JT5) would be more closely related to 3 A. sativa lines than a fellow an A. sterlis line. JT0 and JT5 could be an interesting provider of a genetic control of the resistance to oat crown rust. A crossing between the spontaneous accessions and the differential lines needs to be done to conclude about the originality of the resistance genetic control in JT0 and JT5.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.17(2) [April 2018]|
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