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Title: De novo shoot regeneration from root cultures of Garcinia indica Choiss
Authors: Deodhar, Swapna R
Thengane, R J
Thengane, S R
Keywords: Garcinia indica;Guttifereae;Roots;Shoots;Organogenesis
Issue Date: Jun-2008
Publisher: CSIR
Abstract: Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 µM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 µM), IAA (5.71 µM) and kinetin (4.65 µM). It was observed that shoot length increased to1-2 cm. WPM medium supplemented with NAA (2.69-10.74 µM) and IBA (4.90 µM) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants.
Page(s): 482-486
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.46(06) [June 2008]

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