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dc.contributor.authorDevi, P Uma-
dc.contributor.authorUtsumi, H-
dc.contributor.authorTakata, M-
dc.contributor.authorTakeda, S-
dc.identifier.issn0975-1009(Online); 0019-5189(Print)-
dc.description.abstractWithaferin A (WA), a plant withanolide, has shown significant radiosensitizing effect in vitro and in vivo. Inhibition of DNA repair has been suggested as a mechanism of radiosensitization by WA. To test this, the effect of withaferin A on survival of DT40 chicken B-lymphocyte cell line and its repair deficient single gene mutants Rad54-/-, Ku70-/- and double mutant Ku70-/- /Rad54-/- after irradiation was studied . Exponentially growing cells were treated for 1 hr with 5 mM WA and then exposed to different doses of X-rays. Cell survival was studied by clonogenic assay. WA significantly reduced survival of DT40, Ku70-/- and Ku70-/- /Rad54-/-, but not Rad54-/- cells, suggesting that WA enhances radiosensitivity by interfering with homologous repair, the major pathway of DSB repair in these cells. Inhibition of DNA repair is further indicated in a significant decrease in surviving fraction of DT40 cells by post-irradiation incubation with WA. This could have relevance to cancer radiotherapy.en_US
dc.sourceIJEB Vol.46(06) [June 2008]en_US
dc.subjectChicken B-lymphocytesen_US
dc.subjectDNA repairen_US
dc.subjectSurviving fractionen_US
dc.subjectWithaferin Aen_US
dc.titleEnhancement of radiation induced cell death in chicken B lymphocytes by withaferin Aen_US
Appears in Collections:IJEB Vol.46(06) [June 2008]

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