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Title: Anti-nephrolithiatic potential and the protective role of saponin-rich extract of Dianthus basuticus against acetaminophen-induced damage in HEK293 cells
Authors: Nafiu, Mikhail Olugbemiro
Ashafa, Anofi Omotayo Tom
Sabiu, Saheed
Keywords: Calcium oxalate;Dianthus basuticus;HEK293 cell;Hoechst 33342;MTT;Nephrolithiasis
Issue Date: Jun-2018
Publisher: NISCAIR-CSIR, India
IPC Code: Int. cl. (2015.01)− A61K 36/00, 36/36; A61P 13/00, 39/00
Abstract: Saponins have found ethnomedicinal applications against a number of diseases including oxidative stress, urinary and kidney ailments. This study investigated antioxidant, anti-nephrolithiatic and nephroprotective properties of saponin-rich extract of Dianthus basuticus in vitro. While nephroprotective activity was demonstrated against acetaminophen-mediated toxicity in HEK293 cells using the MTT method, its inhibition of calcium oxalate (CaOx) nucleation was evaluated in the anti-nephrolithiatic study. The antioxidant effect was demonstrated against OH and lipid peroxide radicals. The significant (p <0.05) acetaminophen-induced reduction of viable cells in the nephrotoxic cells following 24 hours incubation was reverted and cell mortality dropped from 81.98 to 13.15 % at 125 µg/mL dose of the extract.  The extract significantly inhibited CaOx nucleation crystals in a dose-related manner with the highest dose being 51.11 % potent. The inhibitory effect of the extract had an overall IC50 of 1.03 µg/mL (R2= 0.9698) which is corroborated by the degree of turbidity of the treated crystals solutions. The overall effects elicited by the extract may be attributed to its antioxidant activity which was concentration-related with the highest dose exhibiting the most potent effect (IC50: 0.45–0.63 µg/mL) when compared with vitamin C (IC50: 0.49–0.69 mg/mL). Hence, D. basuticus is potentially a new lead agent in the management of nephrolithiasis and renal disorders.
Page(s): 117-125
ISSN: 0976-0512 (Online); 0976-0504 (Print)
Appears in Collections:IJNPR Vol.9(2) [June 2018]

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