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Title: In vitro adventitious plant regeneration of Echium orientale L., an endemic plant: The evaluation of biological activities and phenolic content
Authors: Turker, Arzu Ucar
Yildirim, Arzu Birinci
Taş, İsa
Keywords: Antibacterial;Antioxidant;Antitumor;Echium orientale;HPLC;In vitro culture;Micropropagation
Issue Date: Aug-2018
Publisher: NISCAIR-CSIR, India
Abstract: Echium orientale L. is a Turkish endemic plant in the family Boraginaceae. The aim of this study was to develop an efficient in vitro culture protocol for E. orientale and compare the field-grown and in vitro-grown leaves in terms of antibacterial, antitumor and antioxidant activities and phenolic profiles. Leaf and petiole explants were cultured on Murashige & Skoog (MS) medium with various plant growth regulator combinations. Best shoot proliferation was obtained from leaf explant with 1 mg/L thidiazuron (TDZ) and 0.5 mg/L indole-3-acetic acid (IAA), producing 8.7 shoots per explant at 100% shoot frequency. Regenerated shoots were transferred to rooting media containing different levels of IAA, indole-3-butyric acid (IBA), naphthalene acetic acid (NAA) or 2,4 dichlorophenoxyacetic acid (2,4-D). The best root formation was observed with MS basal medium (control). Rooted explants were transferred to vermiculate in Magenta containers for acclimatization and after 3 weeks they were transferred to plastic pots containing potting soil and maintained in the plant growth room. Leaves from field-grown plants showed higher antibacterial, antitumor and antioxidant activities, and phenolic content than those from in vitro-grown plants.
Page(s): 264-272
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.55(4) [August 2018]

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