Genetic stable mass propagation of <em>Acacia mangium</em> Willd. from mature plus tree

Abstract

The study describes an efficient, reproducible and stable <em>in vitro</em> propagation protocol for <em>Acacia mangium</em> from nine years old phenotypically superior plus tree. For effective control of contamination explants were dipped in absolute alcohol for 1 minute followed by 0.1% mercuric chloride solution for 6 minutes. Maximum establishment (87.8%) and shoot proliferation was achieved with an average value of 5.7 shoots per explants and shoot length of 2.8 cm in Murashige & Skoog (MS) media supplemented with 6.66 µM 6-benzylaminopurine (BAP) and 0.465 µM kinetin. The addition of 9.80 µM indolebutyric acid (IBA) in half MS media gave maximum rooting (88.3%) with average 2.7 roots per microshoots with longest root of 18 cm. Rooted plants were hardened and successfully established in the soil. Random amplified polymorphic DNA (RAPD) profile of micropropagated plants shown no change in genetic fidelity up to four growth cycles.