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Title: Standardization of protocol for genomic DNA isolation in Melia composita Benth. (S. M. dubia Cav.)
Authors: Sharma, Shruti
Kumar, Ashok
Dobhal, Shivani
Rawat, Anita
Keywords: Melia composite;DNA extraction;Inter simple sequence repeat (ISSR);Simple sequence repeat (SSR)
Issue Date: Jan-2018
Publisher: NISCAIR-CSIR, India
Abstract: A simple, rapid and efficient method for extraction of genomic DNA from young leaves of Melia composita Benth. (Syn. M dubia Cav.) was optimized for obtaining fine quality DNA. The yield of extracted DNA ranged from 118.40 to 3192.40 µg /500 mg of tissue with absorbance ratio (A260/A280) ranging from 1.41 to 1.96. The quality of extracted DNA was also evaluated by agarose gel electrophoresis which showed a single intact band. The isolated DNA was free of any contaminating proteins, polysaccharides and coloured pigments and successfully amplify inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers.
Page(s): 185-188
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.17(1) [January 2018]

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