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|Title:||Standardization of protocol for genomic DNA isolation in Melia composita Benth. (S. M. dubia Cav.)|
|Keywords:||Melia composite;DNA extraction;Inter simple sequence repeat (ISSR);Simple sequence repeat (SSR)|
|Abstract:||A simple, rapid and efficient method for extraction of genomic DNA from young leaves of Melia composita Benth. (Syn. M dubia Cav.) was optimized for obtaining fine quality DNA. The yield of extracted DNA ranged from 118.40 to 3192.40 µg /500 mg of tissue with absorbance ratio (A260/A280) ranging from 1.41 to 1.96. The quality of extracted DNA was also evaluated by agarose gel electrophoresis which showed a single intact band. The isolated DNA was free of any contaminating proteins, polysaccharides and coloured pigments and successfully amplify inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.17(1) [January 2018]|
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