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IJEB Vol.46(05) [May 2008] >


Title: In vivo visualization of Bonghan ducts inside blood vessels of mice by using an Alcian blue staining method
Authors: Yoo, Jung Sun
Kim, Min Su
Ogay, Vyacheslav
Soh, Kwang-Sup
Keywords: Acupuncture meridian
Alcian blue
Bonghan duct
Hematoxylin and eosin
Hyaluronic acid
Issue Date: May-2008
Publisher: CSIR
Abstract: An in vivo method using Alcian blue (AB) was developed for visualizing floating threadlike tissues inside blood vessels of mice. These novel structures called intravascular Bonghan ducts (IBHDs) are considered as extension of acupuncture meridians. For in vivo imaging of IBHDs, AB solution (pH 7.4) that stains mucopolysaccharides like hyaluronic acid was used. After injecting AB solution into the femoral vein of a mouse, the threadlike structures, stained deep blue, inside the inferior vena cava. The histological results, such as hematoxylin, eosin, and AB staining, show the compositions of the cells and the extracellular matrix in the IBHD. Further studies are needed to investigate their physiological functions, especially in relation with those of other circulatory systems.
Page(s): 336-339
ISSN: 0019-5189
Source:IJEB Vol.46(05) [May 2008]

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