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Title: Antimicrobial protein from Streptomyces fulvissimus inhibitory to methicillin resistant Staphylococcus aureus
Authors: Malik, Himadri
Sur, Banani
Singhal, Neha
Bihari, Vinod
Keywords: Antimicrobial protein
Glycopeptide antibiotics
Methicillin resistant Staphylococcus aureus
Streptomyces fulvissimus
Issue Date: Apr-2008
Publisher: CSIR
Abstract: Fermented culture of Streptomyces fulvissimus was found to secrete an antibacterial protein inhibitory to Micrococcus luteus, Bacillus subtilis, Bacillus cereus and methicillin resistant Staphylococcus aureus (MRSA) strains. The extracellular protein from the fermented culture on concentration revealed a high molecular weight peptide of 63kDa on SDS-PAGE gel and the region on gel displayed inhibitory activity against methicillin resistant Staphylococcus aureus. Bioactivity of the extra cellular protein was non-sensitive to proteinase K, ⍺ chymotrypsin, protease, EDTA (ethylene diamine tetra acetic acid), PMSF (phenyl methyl sulfonyl fluoride) and DMSO (dimethyl sulfoxide) but partially susceptible to amylase and heat. Glycoprotein nature of the proteinaceous compound was confirmed by periodic acid schiff’s (PAS) staining. The secretary protein of S. fulvissimus demonstrated a significant activity against MRSA strain. It could be an important source for developing new drugs to control multidrug resistant gram positive bacteria .
Description: 254-257
ISSN: 0019-5189
Appears in Collections:IJEB Vol.46(04) [April 2008]

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