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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.46 [2008] >
IJEB Vol.46(04) [April 2008] >
| Title: | Antimicrobial protein from Streptomyces fulvissimus inhibitory to methicillin resistant Staphylococcus aureus |
| Authors: | Malik, Himadri
Sur, Banani
Singhal, Neha
Bihari, Vinod |
| Keywords: | Antimicrobial protein
Glycopeptide antibiotics
Methicillin resistant Staphylococcus aureus
SDS–PAGE
Streptomyces fulvissimus |
| Issue Date: | Apr-2008 |
| Publisher: | CSIR |
| Abstract: | Fermented culture of Streptomyces fulvissimus was found to secrete an antibacterial protein inhibitory to Micrococcus luteus, Bacillus subtilis, Bacillus cereus and methicillin resistant Staphylococcus aureus (MRSA) strains. The extracellular protein from the fermented culture on concentration revealed a high molecular weight peptide of 63kDa on SDS-PAGE gel and the region on gel displayed inhibitory activity against methicillin resistant Staphylococcus aureus. Bioactivity of the extra cellular protein was non-sensitive to proteinase K, ⍺ chymotrypsin, protease, EDTA (ethylene diamine tetra acetic acid), PMSF (phenyl methyl sulfonyl fluoride) and DMSO (dimethyl sulfoxide) but partially susceptible to amylase and heat. Glycoprotein nature of the proteinaceous compound was confirmed by periodic acid schiff’s (PAS) staining. The secretary protein of S. fulvissimus demonstrated a significant activity against MRSA strain. It could be an important source for developing new drugs to control multidrug resistant gram positive bacteria . |
| Page(s): | 254-257 |
| ISSN: | 0019-5189 |
| Source: | IJEB Vol.46(04) [April 2008]
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