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Title: Immunomodulatory activity of Neolamarckia cadamba (Roxb.) Bosser with reference to IL-2 induction
Authors: Khandelwal, Vishal
Choudhary, Pradeep Kumar
Goel, Anjana
Bhatia, AK
Gururaj, K
Gupta, Saurabh
Singh, SV
Keywords: RT-PCR;Interleukin-2;Humoral immune response
Issue Date: Jul-2018
Publisher: NISCAIR-CSIR, India
IPC Code: Int. Cl.8: A61K 36/00, A61K 38/20, C12N 15/24, C12N 15/25, C12N 15/26, C12N 15/117, c07K 14/54
Abstract: Neolamarckia cadamba (Roxb.) Bosser syn. Anthocephalus cadamba (Roxb.) Miq., an evergreen tropical tree, known in Hindu Mythology, for its various medical properties. In the present study, immunomodulatory potential of N. cadamba has been investigated in Wistar albino rat model. The effect of hot aqueous extract (HAE) of N. cadamba leaves over differential leukocyte count and humoral immune response was assessed using four groups of six animals each (Gp-I as control, Gp-II orally fed with 125 mg/kg body weight, Gp-III orally fed with 250 mg/kg body weight and Gp-IV orally fed with 500 mg/kg body weight of HAE of N. cadamba leaves). Differential leukocyte count (DLC) was measured in blood, collected from retro-orbital plexus of rats of control and experimental groups. In vivo humoral immune response was determined by estimating the serum antibody titer against Salmonella typhimurium ‘O’ antigen using indirect ELISA. Interleukin (IL)-2 was assayed by sandwich ELISA in presence of different concentrations of HAE (20-500 µg/mL) in the culture supernatant of splenocytes and its expression was determined by quantitative reverse transcription real-time PCR (qRT-PCR). Results suggested significant increase (p < 0.01) in lymphocytes (%) of animals orally fed with different concentrations of HAE of N. cadamba. Serum antibody titer was also significantly increased (p < 0.05) in N. cadamba fed animals. IL-2 level was augmented significantly (p < 0.01) in Concanavalin A (Con A) stimulated in vitro splenocytes culture of 250 µg/mL and 500 µg/mL HAE treated animals in comparison to controls. IL-2 expression was confirmed at molecular level by qRT-PCR analysis of mRNA transcripts of IL-2 gene. Fold expression of IL-2 gene was 28.84 and 330.84 at 250 µg/mL, 500 µg/mL concentrations of HAE respectively in comparison to control. It is concluded that HAE of N. cadamba leaves is a promising drug with immuno-stimulant properties.
Page(s): 451-459
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Appears in Collections:IJTK Vol.17(3) [July 2018]

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