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Title: µCT analysis reveals that Cissus quadrangularis L. stem and Trigonella foenum-graecum L. seed extracts prevent diabetic osteopathy
Authors: Gupta, Madhu
Arshad, Md
Shivnath, Neelam
Khan, Md Sajid
Gupta, Rahul
Keywords: Type I diabetes;Osteopenia;Diabetic osteopathy;Micro-computed tomography (µCT) scan;Cissus quadrangularis L.;Trigonella foenum-graecum L.
Issue Date: Jul-2018
Publisher: NISCAIR-CSIR, India
IPC Code: Int. Cl.8: A61K 36/00, A01D 16/02, A61 17/58, A61K 39/395, A01D 9/00, A61K 45/06
Abstract: Cissus quadrangularis L. (hadjod) and Trigonella foenum-graecum L. (fenugreek) plant parts are used for fracture healing and as an antidiabetic agent, respectively in traditional medicine. This study aimed to investigate the efficacy of C. quadrangularis stem bark extract (CQ) and T. foenum-graecum seed extract (TFG) therapy for the treatment of type I diabetes induced osteopenia. Alloxan (dissolved in 0.1 M citrate buffer (pH = 4.5); intraperitoneally injected @ 130 mg/kg body weight) induced type I diabetic rats were treated with individual and combined doses of ethanol extracts of CQ and TFG for consecutive 30 days @ 250 and 500 mg per kg body weight, orally. Insulin (0.5 IU/kg body weight) was chosen as standard. Body weight and fasting blood glucose were recorded. Two-dimensional (2D) and three-dimensional (3D) analysis of the femur and tibia were performed using micro-computed tomography (µCT) scan to measure histomorphometric parameters of trabecular and cortical regions and bone mineral density (BMD). Elevated level of fasting blood glucose was restored to the vehicle-treated group after treatment with CQ and TFG. Both the extracts significantly improved trabecular histomorphometric parameters, viz. BMD, percent bone volume, trabecular number, trabecular separation, connection density. Cortical mean total cross-sectional bone area, cross-sectional thickness, mean total cross-sectional tissue area were comparable to the vehicle-treated group. CQ and TFG combination therapy showed a marked improvement in bone microarchitecture and can be given together as a potential alternative medicine to insulin for prevention of type I diabetes induced osteopathy. Therefore, it can be considered in the management of diabetic osteopathy in humans.
Page(s): 460-467
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Appears in Collections:IJTK Vol.17(3) [July 2018]

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