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Title: Cloning of an ovule specific promoter from Arabidopsis thaliana and expression of β-glucuronidase
Authors: Nain, Vikrant
Verma, Anju
Kumar, Neeraj
Sharma, Priyanka
Ramesh, B
Kumar, P Ananda
Keywords: AGAMOUS;Ovule;Transcription factor;Transgenics;Tissue specific promoter
Issue Date: Apr-2008
Publisher: CSIR
Abstract: Tissue specific expression of transgenes in plant species has several advantages over constitutive expression. Identification of ovule specific promoters would be useful in genetic engineering of plants with a variety of desirable traits such as genetically engineered parthenocarpy, female sterile plants or seedless fruits. Relative inaccessibility and difficulty in harvesting adequate amounts of tissue at known developmental stages has impeded the progress in cloning of promoters involved in ovule development. In the present study an ovule specific promoter was cloned from Arabidopsis AGL11Ψ gene and used to express GUS (β-glucuronidase) gene in transgenic Arabidopsis. Histochemical staining of GUS appeared in the center of young ovary (ovules), but no detectable GUS activity was observed in vegetative plant tissues, sepals, petals and androecium. AGL11 gene promoter can be useful to modify the developmental path of plants by expressing either plant hormones or lethal genes for agronomic purpose.
Page(s): 207-211
ISSN: 0019-5189
Appears in Collections:IJEB Vol.46(04) [April 2008]

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