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Title: Nutritional regulation of actinomycin-D production by a new isolate of Streptomyces sindenensis using statistical methods
Authors: Praveen, Vandana
Tripathi, Divya
Tripathi, C K M
Bihari, Vinod
Keywords: Actinomycin-D
statistical medium optimization
Issue Date: Feb-2008
Publisher: CSIR
Abstract: Production of actinomycin-D, by an isolate, S. sindenensis, was optimized by statistical methods. Fructose peptone and NaNO3 were found to be critical for antibiotic production. In the second step, their concentrations were optimized with Central Composite Design and Response Surface Methodology. Fructose, peptone and NaNO3 at 2.55, 0.309 and 0.114 % respectively gave ~261 % higher yield (289 mg/l). Cultivation in fermentor at 600 rpm agitation and 1.5 vvm aeration with optimized medium gave 3.56 folds higher yield (365 mg/l) as compared to the yields in shake flasks using normal production medium (80 mg/l).
Description: 138-144
ISSN: 0019-5189
Appears in Collections:IJEB Vol.46(02) [February 2008]

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