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Title: In vitro primary culturing of cells and explant tissue of Conus cumingii venom duct: Cytotoxicity assessment of their culture supernatant on HEK 293T
Authors: Viswanathan, M.S.
Shekar, Mootapally Chandra
Arularasan, Sabapathi
Nathani, Neelam M.
Arumugam, Sathishkumar
Patel, Amrutlal K.
Gadhvi, Indra R.
Joshi, Chaitanya G.
Keywords: Marine cone snail;Venom duct;Cell suspension and Explant tissue;Primary culture;Secretory cells;Cytotoxicity
Issue Date: May-2018
Publisher: NISCAIR-CSIR, India
Abstract: In present study, we attempted to establish primary culture of cells in suspension and tissue explants from the venom duct of Conus cumingii for ample production of conopeptides.  Venom secretary cells migrated from tissue explant within a day and the primary culture cells were successfully maintained up to 20 days. Further, the culture supernatant from both the cell suspension and explant was treated with HEK 293T cells for determining its cytotoxic effects which revealed significant reduction of cell viability giving a prompt evidence for the successful conopetides production by the primary culture in the growth medium.
Page(s): 1096-1102
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.47(05) [May 2018]

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