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|Title:||In vitro primary culturing of cells and explant tissue of Conus cumingii venom duct: Cytotoxicity assessment of their culture supernatant on HEK 293T|
Shekar, Mootapally Chandra
Nathani, Neelam M.
Patel, Amrutlal K.
Gadhvi, Indra R.
Joshi, Chaitanya G.
|Keywords:||Marine cone snail;Venom duct;Cell suspension and Explant tissue;Primary culture;Secretory cells;Cytotoxicity|
|Abstract:||In present study, we attempted to establish primary culture of cells in suspension and tissue explants from the venom duct of Conus cumingii for ample production of conopeptides. Venom secretary cells migrated from tissue explant within a day and the primary culture cells were successfully maintained up to 20 days. Further, the culture supernatant from both the cell suspension and explant was treated with HEK 293T cells for determining its cytotoxic effects which revealed significant reduction of cell viability giving a prompt evidence for the successful conopetides production by the primary culture in the growth medium.|
|ISSN:||0975-1033 (Online); 0379-5136 (Print)|
|Appears in Collections:||IJMS Vol.47(05) [May 2018]|
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