Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/4409
Title: Detection of <i style="">in vitro</i> and <i style="">in vivo</i> released antigens of diagnostic interest in <i style="">Mycobacterium tuberculosis </i>by immunoblotting<i style=""></i>
Authors: Shende, Niraj
Gupta, Sonika
Upadhye, Vijay
Kumar, Satish
Harinath, Bhaskar C
Keywords: <i style="">In vitro</i> and <i style="">in vivo </i>released antigens
Immunoblotting
Pulmonary tuberculosis
Issue Date: Jan-2008
Publisher: CSIR
Abstract: Identification of <i style="">in vitro</i> and <i style="">in vivo</i> released mycobacterial antigens are of considerable interest in diagnosis of <i style="">Mycobacterium tuberculosis. </i>Isolation of <i style="">in vitro </i>released antigen from<i style=""> M. tb</i> excretory-secretory culture filtrate protein and <i style="">in vivo</i> released circulating tuberculous antigen from smear positive pulmonary tuberculosis sera by ammonium sulphate precipitation is reported. The antigens were resolved by SDS–PAGE and immunoblotting was performed using pooled serum of smear positive, smear negative pulmonary tuberculosis sera and normal sera to identify reactive tuberculous antigens. <i style="">In vitro </i>and <i style="">in vivo </i>released mycobacterial antigens showed reactivity at 100, 31, 43 and 20 kDa with smear positive and smear negative pulmonary tuberculosis patients. Further, the <i style="">in vitro</i> released antigen showed strong reactivity exclusively at 55 kDa antigen with smear positive and 24 kDa antigen with smear negative pulmonary tuberculosis sera. <i style="">In vivo </i>released antigen reacted exclusively at 170 and 16 kDa with smear positive and 19 kDa antigen with smear negative pulmonary tuberculosis patients. Antigens of 24 and 19 kDa which are reactive with sputum negative sera will be of diagnostic interest and need further study in patients with low bacillary load. The <i style="">in vitro</i> and <i style="">in vivo</i> released mycobacterial 100, 31, 43 and 20 kDa antigens, reactive with patients sera are of diagnostic interest in tuberculosis.
Description: 18-21
URI: http://hdl.handle.net/123456789/4409
ISSN: 0019-5189
Appears in Collections:IJEB Vol.46(01) [January 2008]

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