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Title: Comparison of performance of two DNA line probe assays for rapid detection of multidrug-resistant isolates of <i style="">Mycobacterium tuberculosis</i>
Authors: Ahmad, Suhail
Al-Mutairi, Noura M
Mokaddas, Eiman
Keywords: GenoType MTBDR
Line probe assays
Multidrug resistance
<i style=""> Mycobacterium tuberculosis</i>
Issue Date: Jun-2009
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place" downloadurl=""><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="metricconverter"> Infections with multidrug-resistant (resistant at least to rifampicin, RIF and isoniazid, INH) strains of <i style="">Mycobacterium tuberculosis</i> (MDR-TB) are associated with high case fatality rates. Rapid identification of MDR-TB strains is important for early institution of appropriate therapy. Two DNA line probe assays, GenoType MTBDR (GT-MTBDR) and INNO-LiPA Rif. TB (INNO-LiPA) were compared for their abilities to detect resistance to INH and RIF in 80 <i style="">M</i><i style="">. tuberculosis</i> isolates. The test results were compared to those obtained by conventional drug susceptibility testing (DST), DNA sequencing and/or PCR-restriction fragment length polymorphism (RFLP) analysis of regions of interest of <i style="">M. tuberculosis</i> genome. Compared to the DST and <i style="">katG</i> codon 315 PCR-RFLP results, GT-MTBDR test results were concordant for INH resistance for 63 of 80 (78.7%) isolates. For RIF resistance, GT-MTBDR and INNO-LiPA test results were concordant with DST for 74 of 80 (92.5%) and 76 of 80 (95%) strains, respectively. The GT-MTBDR test results correlated with sequencing results for 77 of 80 (96.2%) while INNO-LiPA results for 79 of 80 (98.7%) isolates. Both the tests are useful for rapid detection of MDR-TB strains, however, GT-MTBDR assay offers the advantage of detecting the resistance to both INH and RIF simultaneously when MDR-TB is suspected. </smarttagtype></smarttagtype>
Description: 454-462
ISSN: 0019-5189
Appears in Collections:IJEB Vol.47(06) [June 2009]

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