NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.47 [2009] >
IJEB Vol.47(06) [June 2009] >

Title: Comparison of performance of two DNA line probe assays for rapid detection of multidrug-resistant isolates of Mycobacterium tuberculosis
Authors: Ahmad, Suhail
Al-Mutairi, Noura M
Mokaddas, Eiman
Keywords: GenoType MTBDR
Line probe assays
Multidrug resistance
Mycobacterium tuberculosis
Issue Date: Jun-2009
Publisher: CSIR
Abstract:  Infections with multidrug-resistant (resistant at least to rifampicin, RIF and isoniazid, INH) strains of Mycobacterium tuberculosis (MDR-TB) are associated with high case fatality rates. Rapid identification of MDR-TB strains is important for early institution of appropriate therapy. Two DNA line probe assays, GenoType MTBDR (GT-MTBDR) and INNO-LiPA Rif. TB (INNO-LiPA) were compared for their abilities to detect resistance to INH and RIF in 80 M. tuberculosis isolates. The test results were compared to those obtained by conventional drug susceptibility testing (DST), DNA sequencing and/or PCR-restriction fragment length polymorphism (RFLP) analysis of regions of interest of M. tuberculosis genome. Compared to the DST and katG codon 315 PCR-RFLP results, GT-MTBDR test results were concordant for INH resistance for 63 of 80 (78.7%) isolates. For RIF resistance, GT-MTBDR and INNO-LiPA test results were concordant with DST for 74 of 80 (92.5%) and 76 of 80 (95%) strains, respectively. The GT-MTBDR test results correlated with sequencing results for 77 of 80 (96.2%) while INNO-LiPA results for 79 of 80 (98.7%) isolates. Both the tests are useful for rapid detection of MDR-TB strains, however, GT-MTBDR assay offers the advantage of detecting the resistance to both INH and RIF simultaneously when MDR-TB is suspected.
Page(s): 454-462
ISSN: 0019-5189
Source:IJEB Vol.47(06) [June 2009]

Files in This Item:

File Description SizeFormat
IJEB 47(6) 454-462.pdf442.63 kBAdobe PDFView/Open
 Current Page Visits: 747 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 169672 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: