Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/4363
Title: Role of cholesterol in<i style=""> Mycobacterium tuberculosis </i>infection
Authors: Miner, Maurine D
Chang, Jennifer C
Pandey, Amit K
Sassetti, Christopher M
Sherman, David R
Keywords: Cholesterol
Δigr
Lipid metabolism
<i style="">mce4</i>
<i style="">Mycobacterium tuberculosis</i>
Issue Date: Jun-2009
Publisher: CSIR
Abstract: <i style="">Mycobacterium tuberculosis</i> (MTB) acquisition and utilization of nutrients within the host cell is poorly understood, although it has been hypothesized that host lipids probably play an important role in MTB survival. Cholesterol has recently been identified as an important lipid for mycobacterial infection. The <i style="">mce4</i> transport system is required for cholesterol import into bacterial cells, and deletion of <i style="">mce4</i> locus resulted in severe attenuation in a chronic mouse model of infection. However, it has remained unclear what additional bacterial functions were required for utilization of this sterol. We have found that the <i style="">igr</i> locus, which was previously found essential for intracellular growth and virulence of MTB, is required for cholesterol metabolism: <i style="">igr</i>-deficient bacteria cannot grow using cholesterol as a primary carbon source. The growth-inhibitory effect of cholesterol <i style="">in vitro</i> depends on cholesterol import, as the Δ<i style="">igr</i> mutant growth defect during the early phase of disease is completely suppressed by mutating <i style="">mce4</i>, implicating cholesterol intoxication as the primary mechanism of attenuation. We conclude that <i style="">M. tuberculosis</i> metabolizes cholesterol throughout the course of infection, and that degradation of this sterol is crucial for bacterial persistence.
Description: 407-411
URI: http://hdl.handle.net/123456789/4363
ISSN: 0019-5189
Appears in Collections:IJEB Vol.47(06) [June 2009]

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