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|Title:||Survival and changes in morphology, mitotic and metabolic activity of yeast Candida guilliermondii exposed to X-irradiation|
|Abstract:||The investigation of survival and vital activity of lower eukaryotes – yeasts has a great interest under the influence of X-radiation because yeasts are widely used in radiation biology; they are convenient model for investigating of radiation-induced inactivation and further repair mechanisms of higher eukaryotes, and the mechanisms of X-rays influence on them are still unclear. The investigation of survival and vital activity of yeasts Candida guilliermondii exposed to X-irradiation has shown that the yeasts were characterized by the sigmoid curve of survival, and the LD50 was 720 Gy. After X-irradiation their growth kinetics was delayed on 2 h in comparison with non-irradiated yeasts, the ability of colony forming units in stationary growth phase was decreased approx. 3 fold. In the population of X-irradiated yeasts fibered and giant cells appeared, as revealed by electron microscopy. The vitality of non-irradiated yeasts was 56%, X-irradiated yeasts did not have the ability for fermentation, and after post-radiation incubation, and the vitality of yeasts was partially recovered; although it was significantly lower than in non-irradiated yeasts (9.7%). Thus, X-irradiation resulted the fall of mitotic and metabolic activity of yeasts. The obtained data can be used in radiation biology for developing methods of anti-radiation protection of living organisms.|
|ISSN:||0975-0959 (Online); 0301-1208 (Print)|
|Appears in Collections:||IJBB Vol.54(6) [December 2017]|
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