Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/43335
Title: Genetic diversity in Oroxylum indicum (L.) Vent., a threatened medicinal plants from India by ISSR analysis
Authors: Rajasekharan, P E
Kareem, V K Abdul
Ravish, B S
Mini, S
Keywords: Genetic diversity;Genetic structure;ISSR;Oroxylum indicum
Issue Date: Jul-2017
Publisher: NISCAIR-CSIR, India
Abstract: Oroxylum indicum is an important threatened traditional medicinal plant native to Indian subcontinent. Inter Simple Sequence Repeats (ISSR) markers were used to assess the genetic diversity and population genetic structure of 39 accessions of O. indicum belonging to South and North East India. A total of 92 discernible bands were generated from 17 primers, with 81 (88.04%) being polymorphic, indicating high genetic diversity at the species level. The Nei’s gene diversity (h) was estimated to be 0.2526 within populations, and 0.4027 at the species level. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly among populations (68%), but variance within populations was only 32%. In addition, Nei’s differentiation coefficients (GST) was found to be high (0.3733), confirming the relatively high level of genetic differentiation among populations. The unweighted pair group method of arithmetic-average (UPGMA) clustering also demonstrated high genetic differentiation between South and North East Indian populations. The estimated gene flow (Nm) from GST was 0.8393. It indicated that the fragmentation of populations and anthropogenic activities might result in specific evolutionary history. Based on the genetic data, a conservation strategy is proposed for this threatened species.
Page(s): 357-365
URI: http://nopr.niscair.res.in/handle/123456789/43335
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.16(3) [July 2017]

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