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|Title:||Isolation and partial characterization of cellulase free alkalo-thermophilic xylanase produced by Bacillus flexus|
|Authors:||Bora, Sudipta Sankar|
Lahan, Jyoti Prasad
|Abstract:||Bacillus flexus, an alkalophilic bacterial strain, was isolated from tea rhizosphere of GolaghatDistrict of Assam by culture enrichment method. During the study the strain was found to produce cellulose-free xylanase of molecular weight 25 kDa which was determined using SDS-PAGE analysis. The enzyme had high hydrolytic activity against birchwoodxylan, oat spelt xylan and beech wood xylan; and exhibited relatively low activity against cellobiose, starch, carboxy methyl cellulose and avicel. Optimum PH and temperature in which enzyme was found active at pH 8.0 ± 0.2 and at 70°C ± 2°C respectively. The Km and Vmax values were found to be 0.9 mg/ml and 3633 U/ml, respectively. Hydrolytic activity of the enzyme with metal ions were also determined and interestingly it was found that Co2+, Zn2+, Mg2+, Fe3+ and Ca2+ elevated the enzyme activity while Hg2+ and metal chelator EDTA reduced its activity.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.16(3) [July 2017]|
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