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Title: Enzyme and free radical inhibitory potentials of ethyl acetate extract of endophytic actinomycete from Syzygium cumini
Authors: Saini, Preeti
Gangwar, Madhurama
Keywords: α-Amylase;α-Glucosidase;Hydroxyl radical;Kinetics;Reducing power;Total phenol
Issue Date: Oct-2017
Publisher: NISCAIR-CSIR, India
Abstract: Endophytic actinomycete, J-8 was obtained from the shoot tissues of traditional Indian medicinal plant, Syzygium cumini. Based on the biochemical, cultural and morphological analyses the isolate was tentatively identified as Streptomyces albosporus. This strain was cultivated in starch casein broth medium at 28 ± 2°C for 10 days. The crude extract (EAE) obtained with ethyl acetate: methanol (4:1), was dried to powder at −130°C in vacuo. The EAE exhibited 54.48 % and 62.17 % inhibition against alpha-amylase and alpha-glucosidase respectively at 100 µg/mL. It was also able to inhibit diffusion of glucose across dialysis membrane in vitro until a time period of 24 h, at 1000 µg/mL concentration. EAE was observed to have reducing power activity at par with butylated hydroxytoluene. Significantly high hydroxyl radical scavenging activity was observed, with an IC50 value of  23.94 µg/mL. EAE was also having the antioxidant capability (IC50=236.39 µg/mL), as determined by β-carotene linoleate model system. Further, total phenol content was measured to be 38.88 µg/mL, represented as catechol equivalents. The study carried out to determine the mode of inhibition against α-amylase revealed that the inhibition was of near competitive type. From the results, it is evident that the ethyl acetate extract of isolate J-8 has potent antioxidant and enzyme inhibitory activity in vitro.
Page(s): 207-213
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.54(5) [October 2017]

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