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|Title:||Antioxidant and oxidative DNA damage protective properties of leaf, bark and fruit extracts of Terminalia chebula|
|Keywords:||Antioxidant activity;DNA protective activity;Terminalia chebula|
|Abstract:||Terminalia chebula Retz., is an important herbal drug in Indian system of medicine. In the present study, methanol extract and fractions of leaf, bark and fruit of T. chebula were investigated for their antioxidant and DNA protective properties. The antioxidant activities of these extracts and fractions were evaluated by in vitro methods, including, DPPH radical scavenging assay, superoxide radical scavenging assay, hydroxyl radical scavenging assay, reducing power assay and metal chelating assay. DNA protective activity was also determined using hydroxyl radical induced DNA strand scission. Total phenolic content of the extracts and fractions was measured using Folin-Ciocalteu method. Among the different extracts and fractions, highest radical scavenging abilities were obtained in acetone fraction of fruit, including DPPH radical (EC50 value of 9.9 ± 0.70 μg/mL) superoxide anions (EC50 value of 27.0± 2.09 μg/mL), and hydroxyl radicals (EC50 value of 35.6 ± 1.97 μg/mL) scavenging activities. Similarly, reducing power (EC50 value of 71.4 ± 4.30 μg/mL) and percent Fe2+ chelating capacity (70.6 ± 4.84 at 500 μg/mL) was also highest in acetone fraction of fruit. Our results also revealed remarkable ability of the acetone fraction of fruit and bark in protecting the supercoiled pUC18 plasmid DNA against strand scission induced by hydroxyl radicals. In addition, our study showed significant (P <0.01) linear correlation between antioxidant activities and total phenolic content of extracts and fractions.|
|ISSN:||0975-0959 (Online); 0301-1208 (Print)|
|Appears in Collections:||IJBB Vol.54(3&4) [June-August 2017]|
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