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Title: A comparative study on three quantitating methods of microalgal biomass
Authors: Lu, Lin
Yang, Guanpin
Zhu, Baohua
Pan, Kehou
Keywords: Microalgae;Optical density;Cell density;Dry weight
Issue Date: Nov-2017
Publisher: NISCAIR-CSIR, India
Abstract: Nannochloropsis oculata, Nannochloropsis salina, Nannochloropsis oceanica, Phaeodactylum tricornutum and Cylindrotheca fusiformis were cultured in liquid f/2 medium with aeration till the end of exponential growth phase and serially diluted into eight cell suspensions, and cell concentration, optical density and dry weight in each suspension were determined. The cell density was counted on a hemocytometer, while optical density was measured in the spectrophotometer at 750 nm and dry weight was assayed after a lyophilization procedure. We found significant linear correlations between cell density, optical density and dry weight. When the cell concentration was low, the correlation between optical density and cell density was stronger than that between dry weight and cell density. When the cell concentration was high, the dry weight method was more accurate than other two, thus being applicable to single and high quality measurement.
Page(s): 2265-2272
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.46(11) [November 2017]

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