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Title: Impact of high cholesterol diet in mediating inflammation provoked calcinosis in renal tissue of experimental rats
Authors: Rajeswari, R
Divya, J
Jayasudha, E
Thellamudhu, G
Suresh, M
Raman, Thulasi K
Prema, V
Kalaiselvi, P
Keywords: Aging;Brush border;Membrane enzymes;Hypercholesterolemia;Renal stones;Tamm-Horsfall glycoprotein;Urine;Biochemistry
Issue Date: Feb-2017
Publisher: NISCAIR-CSIR, India
Abstract: Several scientific studies suggest the detrimental effects of dietary cholesterol in augmenting renal calcification. However, the scenario behind age associated hypercholesterolemia and renal stone formation is still a mystery. Thus the endeavor of this research was to highlight the impact of high cholesterol diet (HCD) in urolithiasis during aging. Male albino rats of Wistar strain (3 months-young and 24 months old-aged) were used in this study. Hypercholesterolemia was induced by the diet comprising of the normal rat chow supplemented with 4% cholesterol and 1% cholic acid. Determination of urinary stone forming risk factors, membrane damage markers, expression of few lithogenic and anti-lithogenic proteins and renal histopathology were analyzed. The results revealed the elevated concentration of urinary stone forming factors such as uric acid, creatinine, oxalate, calcium, and phosphorus levels, whereas citrate level was decreased in aged hypercholesterolemic rats. Increased risk of stone formation was dictated by the increased expression of albumin and decreased expression of anti-lithogenic Tamm-Horsfall glycoprotein (THP) in the renal tissue of young and aged hyperlipidemic rats. Moreover, increased activities of urinary marker enzymes and brush border membrane enzymes in aged HCD fed rats substantiate renal tubular damage. Histopathological studies confirmed the accretion of lipid and heightening of renal inflammation. These observations validate the role of hypercholesterolemia pertaining to lithiasis during aging. Our analysis also depicts that advanced age is not only risk factor for the formation of kidney stones, but its coexistence with other risk factors such as hypercholesterolemia will make the kidneys more sensitive to renal calculi formation.
Page(s): 71-81
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.54(1&2) [February-April 2017]

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