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Title: Production Characteristics, Activity Patterns and Biodecolourisation Applications of Thermostable Laccases from Corynebacterium Efficiens and Enterobacter Ludwigii
Authors: Odeniyi, O A
Unuofin, J O
Adebayo-Tayo, B C
Wakil, S M
Onilude, A A
Keywords: Corynebacterium Efficiens;Enterobacter ludwigii;Media Optimisation;Laccase Characterisation;Dye-Decolourisation
Issue Date: Sep-2017
Publisher: NISCAIR-CSIR, India
Abstract: The production conditions, characteristics and dye decolourisation potentials of two purified bacterial laccases from Corynebacterium efficiens and Enterobacter ludwigii were investigated. Optimum production pH was 3.0 and 4.0 respectively for E. ludwigii and C. efficiens. Khaya sp. and Gmelina arborea wood shavings, (NH4)2SO4 (30.2µM/ml) and K+ maximally supported laccase production in both organisms. The Vmax and specificity constants for both purified laccases were 44.6µM/mg; 1858.4S-1µM-1and 23.15µM/mg; 1015.4S-1µM-1 respectively. Maximum laccase activity was between 80°C and 90°C and pH 7.0. In both laccases, Fe3+ induced higher activity and red Dylon dye was 69% and 64% decolourised. The properties exhibited by these laccases could enhance their suitability in diverse biotechnological applications.
Page(s): 562-569
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.76(09) [September 2017]

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