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Title: Enhanced environment alters the myelin composition and neurochemistry of fore and mid brain regions in rats subjected to immobilization stress
Authors: Vanisree, AJ
Perumalpillai, Kirijayini
Gangadharan, Thamizhoviya
Keywords: Enhanced environment;Immobilisation stress;Myelin;Neurotransmitters
Issue Date: Oct-2016
Publisher: NISCAIR-CSIR, India
Abstract: Stress is a major factor contributing to various psychological and neurological disorders and hence needs to be addressed by simple remedial measures to mitigate the severity. This study assesses the impact of enhanced environment (EE) on fore and mid brain regions of rats under stress. We used immobilization restraint stress (IS) strategy (4 h) to impart stress in rats and also exposed the rats to EE (1 h) for 21 days. Behavioural changes in the rats, neurotransmitters, cortisol levels and composition of myelin membrane isolated from forebrain (FB) and midbrain (MB) regions were evaluated. The behavioural assessment revealed an impaired state by immobilisation stress (IS) which, however, improved on EE exposure. The observed levels of serotonin, dopamine, glutamate and GABA emphasized the effectiveness of EE significantly (P <0.001). IS had challenged the protein composition of myelin and the activities of membrane bound ATPases, but EE could alter the adverse effect significantly (P <0.001). Interestingly, control rats showed a reduction in the activity of acetylcholinesterase, unlike ATPases and 5′nucleotidase in their MB regions. EE had a significant effect on the altered activities of all these enzymes except that of 5′nucleotidase (P=0.050). The alterations of lipid profile of myelin also highlighted the impact of EE on IS by maintaining almost normal lipid profile (P <0.001). Cortisol was found to be elevated in stress induced group while EE was able to attenuate the levels of cortisol. Our results also suggests that the potential impact of EE on FB was minimal compared to MB. The observed IS+EE–myelin interaction deserves further investigations to validate the significance of myelin in stress biology.
Page(s): 169-178
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.53(5&6) [October-December 2016]

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