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Title: Effect of wood apple<i style=""> Aegle marmelos</i>, Correa (Dicotyledons, Sapindales, Rutaceae) extract as an antibacterial agent on pathogens infecting prawn (<i style="">Penaeus indicus</i>) larviculture
Authors: Citarasu, T.
Rajajeyasekar, R.
Venkatramalingam, K.
Dhandapani, P. S.
Marian, M. Peter
Keywords: <i>Aegle marmelos</i>
<i>Artemia franciscana</i>
<i>Penaeus indicus</i>
Issue Date: Jun-2003
Publisher: CSIR
Abstract: <i style="">Aegle marmelos</i>, an antimicrobial plant was extracted with 100% methanol and the sensitivity of the extract was assessed against different fish diseases caused by pathogenic bacteria. For sensitivity test, the minimal bacterial population growth (10-20<sub> </sub>%) was observed in the 40 mg/100 ml concentration of the culture medium. Different concentrations (25,50,75 and 100<sub> </sub>%) of the extracts were enriched with <i style="">Artemia franciscana</i> nauplii/pre-adult. These were fed to the <i style="">Penaeus indicus</i> post-larvae (6.5 to 6.85 mg of average weight) for 25 days, which were reared with four different pathogens (<i style="">Pseudomonas aeruginosa, Aeromonas hydrophila</i>, <i style="">Salmonella typhi </i>and<i style=""> Vibrio </i>sp<i style="">.</i>) inoculated seawater. Among the four pathogens<i style="">, P.aeruginosa</i> was very sensitive to <i style="">A. marmelos</i> extract. <i style="">Penaeus indicus</i> post larvae fed on the un-enriched <i style="">Artemia</i> exhibited the lowest survival, which averaged 21<sub> </sub>%, and this increased to the maximum of 58<sub> </sub>% (averaged) in the 75<sub> </sub>% extract enriched <i style="">Artemia </i>fed post larvae of <i style="">P.indicus</i>. Similarly, the weight gain and specific growth rate were also increased significantly (P< 0.01) in the enriched <i style="">Artemia</i> fed post-larvae of <i style="">P.indicus</i>. Among the four different pathogens inoculated, the post-larvae, which were reared in the <i style="">P.aeruginosa </i>inoculation<i style="">,</i> exhibited the maximum survival (37.35 mg) and better growth rate (12.03<sub> </sub>%). Also the bacterial count (cfu/g) was significantly (P<0.01) differed from the un-enriched to enriched <i style="">Artemia</i> fed post-larvae. The extract helped to reduce the bacterial count of a maximum of 1.9, 2.45, 0.96 and 2.36 times in the <i style="">P.aerugiosna, A.hydrophila, S.typhi and Vibrio </i>sp. respectively in the 100<sub> </sub>% extract enriched <i style="">Artemia </i>fed post-larvae of <i style="">P.indicus</i>. Among the different percentages of enrichment, 75<sub> </sub>% was considered the best for survival and growth characteristics of the post larvae.
Description: 156-161
ISSN: 0379-5136
Appears in Collections:IJMS Vol.32(2) [June 2003]

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