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IJMS Vol.38(1) [March 2009] >


Title: Detection of genetic variation in four Ulva species based on RAPD technique
Authors: prasad, Umashankar
Deshmukhe, Geetanjali
Dwivedi, Alkesh
Singh, S. D.
Keywords: seaweed
Ulva
DNA isolation
genetic variation
RAPD
Issue Date: Mar-2009
Publisher: CSIR
Abstract: DNA yield was obtained by using 2 protocols, CTAB (Cetyltrimethylammonium bromide) and modified Wattier et al; out of which the latter yielded more quantity of DNA (0.85 mg/g). Out of total amplified product 53% bands were shown monomorphic and remaining of the bands were polymorphic. The high frequency of polymorphic bands suggests that the isolates of Ulva represented in our collection have sufficient genetic diversity for conducting a valid heterosis experiment. The intra species genetic similarity (GS) value was found highest for U. lobata and lowest for U. fasciata. The interspecies GS value was found highest between U. fasciata/U. lactuca and lowest between U. lobata/U. reticulata. The large number of differences among isolates revealed by the RAPD technique indicate that it would be possible to establish a unique “fingerprint” for individual plants based on the combined results generated from a small collection of primers.
Page(s): 52-56
ISSN: 0379-5136
Source:IJMS Vol.38(1) [March 2009]

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