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Title: Detection of genetic variation in four <i>Ulva </i>species based on RAPD technique
Authors: prasad, Umashankar
Deshmukhe, Geetanjali
Dwivedi, Alkesh
Singh, S. D.
Keywords: seaweed
DNA isolation
genetic variation
Issue Date: Mar-2009
Publisher: CSIR
Abstract: DNA yield was obtained by using 2 protocols, CTAB (Cetyltrimethylammonium bromide) and modified Wattier <i>et al</i>; out of which the latter yielded more quantity of DNA (0.85 mg/g). Out of total amplified product 53% bands were shown monomorphic and remaining of the bands were polymorphic. The high frequency of polymorphic bands suggests that the isolates of <i style="">Ulva</i> represented in our collection have sufficient genetic diversity for conducting a valid heterosis experiment. The intra species genetic similarity (GS) value was found highest for <i style="">U. lobata</i> and lowest for <i style="">U. fasciata</i>. The interspecies GS value was found highest between <i style="">U. fasciata/U. lactuca</i> and lowest between <i style="">U. lobata/U. reticulata</i>. The large number of differences among isolates revealed by the RAPD technique indicate that it would be possible to establish a unique “fingerprint” for individual plants based on the combined results generated from a small collection of primers.
Description: 52-56
ISSN: 0379-5136
Appears in Collections:IJMS Vol.38(1) [March 2009]

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