NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Geo-Marine Sciences (IJMS) >
IJMS Vol.38 [2009] >
IJMS Vol.38(1) [March 2009] >

Title: Detection of genetic variation in four Ulva species based on RAPD technique
Authors: prasad, Umashankar
Deshmukhe, Geetanjali
Dwivedi, Alkesh
Singh, S. D.
Keywords: seaweed
DNA isolation
genetic variation
Issue Date: Mar-2009
Publisher: CSIR
Abstract: DNA yield was obtained by using 2 protocols, CTAB (Cetyltrimethylammonium bromide) and modified Wattier et al; out of which the latter yielded more quantity of DNA (0.85 mg/g). Out of total amplified product 53% bands were shown monomorphic and remaining of the bands were polymorphic. The high frequency of polymorphic bands suggests that the isolates of Ulva represented in our collection have sufficient genetic diversity for conducting a valid heterosis experiment. The intra species genetic similarity (GS) value was found highest for U. lobata and lowest for U. fasciata. The interspecies GS value was found highest between U. fasciata/U. lactuca and lowest between U. lobata/U. reticulata. The large number of differences among isolates revealed by the RAPD technique indicate that it would be possible to establish a unique “fingerprint” for individual plants based on the combined results generated from a small collection of primers.
Page(s): 52-56
ISSN: 0379-5136
Source:IJMS Vol.38(1) [March 2009]

Files in This Item:

File Description SizeFormat
IJMS 38(1) 52-56.pdf548.21 kBAdobe PDFView/Open
 Current Page Visits: 762 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 167639 since 01-Sep-2015  Last updated on 28-Jun-2016Webmaster: