Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/4242
Title: Batch culture fermentation of <i>Penicillium chrysogenum </i>and a report on the isolation, purification, identification and antibiotic activity of citrinin
Authors: Devi, Prabha
D'Souza, Lisette
Kamat, Tonima
Rodrigues, Celina
Naik, Chandrakant G.
Keywords: Citrinin
secondary metabolite production
isolation
spectral identification
antibiotic activity
Issue Date: Mar-2009
Publisher: CSIR
Abstract: Batch fermentation of <i>Penicillium</i> <i>chrysogenum</i>, MTCC 5108 was carried out using potato dextrose broth medium prepared in seawater: distilled water (1:1). Biomass as dry weight was determined by gravimetric analysis. Citrinin, the main secondary metabolite, is produced in large quantities during the stationary phase of growth. The yield amounted to approximately 530 mg l<sup>-1</sup>. After optimization of culture conditions, <i>P</i>. <i>chrysogenum</i> was mass cultured and citrinin was isolated and purified from the medium using a combination of chromatographic techniques (Thin layer and column chromatography). Citrinin, thus obtained was characterized on the basis of its spectral data (UV, Proton Nuclear Magnetic Resonance and Electrospray Ionization Mass spectra). The present study consists report on the effect of the concentration of citrinin on the growth of the culture and antibiotic activities assayed by disc diffusion method using clinical pathogens.
Description: 38-44
URI: http://hdl.handle.net/123456789/4242
ISSN: 0379-5136
Appears in Collections:IJMS Vol.38(1) [March 2009]

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