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IJEB Vol.47(05) [May 2009] >


Title: Comparative potential of modified indigenous, indigenous and commercial ELISA kits for diagnosis of Mycobacterium avium subspecies paratuberculosis in goat and sheep
Authors: Singh, A V
Singh, S V
Sohal, J S
Singh, P K
Keywords: Absorbed ELISA
Commercial ELISA
Johne’s disease
Mycobacterium avium
Paratuberculosis
Issue Date: May-2009
Publisher: CSIR
Abstract: In the present study, modified indigenous ELISA kit (kit 1) was compared with indigenous ELISA kit (kit 2) and commercial ELISA kit (kit 3) for its sensitivity and specificity with respect to faecal culture for diagnosis of Johne’s disease in goats and sheep under natural conditions. Of the 64 positive animals, serum of 42.1, 48.4 and 18.7% animals yielded positive infection in kit 1, 2 and 3, respectively. Specificity of kit 1 (95.1%) was maximum followed by kit 3 (93.7%) and kit 2 (83.4%). Kit 1 showed superior diagnostic potential than the other two kits. Kit 1 may be used as single screening test regimen for diagnosis of MAP infection in the population of goats and sheep in India.
Page(s): 379-382
ISSN: 0019-5189
Source:IJEB Vol.47(05) [May 2009]

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