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IJEB Vol.47(05) [May 2009] >


Title: An improved method for staining kinetochores of human chromosomes
Authors: He, Junlin
Liu, Xueqing
Ding, Yubin
Yu, Chao
Weng, Yaguang
Chen, Xuemei
Gao, Rufei
Wang, Yingxiong
Keywords: Chromosomal segregation
Kinetochore
Nucleolar organizer regions
Silver nitrate staining
Issue Date: May-2009
Publisher: CSIR
Abstract: An improved method, which exhibited simultaneously clearly kinetochores and the nucleolar organizer regions of human chromosomes by pretreating of human metaphase chromosomes with HCl and NaOH, followed by staining with silver nitrate and visualizing using ammoniacal silver, is described in the present communication. It has important role for analysis of kinetochore variation, mechanism of chromosomal non-disjunction as well as identification of fuctional active centromeres.
Page(s): 376-378
ISSN: 0019-5189
Source:IJEB Vol.47(05) [May 2009]

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