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Title: A combined approach using RAPD, ISSR and bioactive compound for the assessment of genetic diversity in Aloe vera (L.) Burm.f.
Authors: Kumar, Ravinder
Naik, Pradeep K
Kumar, Anil
Aggarwal, Himanshu
Kumar, Ashok
Chhokar, Vinod
Keywords: Aloe vera;Aloin content;Genetic polymorphism;ISSR;RAPD
Issue Date: Oct-2016
Publisher: NISCAIR-CSIR, India
Abstract: Aloe vera (L.) an important medicinal plant valued all over the world for its pharmacological importance. Despite limited knowledge of the levels of genetic diversity and relatedness, their cultivation as a source of valuable secondary metabolites is widespread. In order to facilitate reasoned scientific decisions on its conservation and for selective breeding programme, aloin content and genetic diversity analysis of 55 genotypes were performed. Aloin content in the leaves of 55 genotypes varied from 3.29 to 276.76 mg/g of dry wt. Twenty six RAPD and fourteen ISSR primers amplified a total of 236 and 111 scorable bands, of which 86.44 and 72.07% were polymorphic, respectively. Analysis of molecular variance (AMOVA) indicated high genetic variation among genotypes. Genetic variation among genotypes grouped into low, intermediate and high aloin content was negligible, 5.4% (RAPD) and 4.08% (ISSR). The dendrogram obtained from Neighbor-joining and STRUCTURE analysis revealed splitting of genotypes into four clusters with no clear distinction between low, intermediate and high aloin content genotypes. Results showed that genetic variability, using RAPD and ISSR, was not associated with aloin content. However, both the markers revealed high genetic variation among genotypes, which is important in the conservation and exploitation of A. vera genetic resources.
Page(s): 538-549
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.15(4) [October 2016]

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