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IJBB Vol.46(2) [April 2009] >


Title: Interactions of bacterial polysaccharides with cationic dyes: Physicochemical studies
Authors: Dasgupta, S
Nath, R K
Biswas, S
Mitra, A
Panda, A K
Keywords: Acridine orange
Bacterial polysaccharide
Binding constant
Dye-polymer interaction
Fluorescence quenching
Klebsiella
Metachromasy
Pinacyanol chloride
Issue Date: Apr-2009
Publisher: CSIR
Abstract: Capsular polysaccharides (SPS) are an integral component of gram-negative bacteria, and also have potential use as vaccine. In this paper, interactions of SPS isolated from Klebsiella strains K20 and K51 with cationic dyes pinacyanol chloride (PCYN) and acridine orange (AO) were studied by absorbance and fluorescence measurements. Both the polysaccharides having glucuronic acid as the potential anionic site induced strong metachromasy (blue shift ~100 nm) in the PCYN. The spectral changes were studied at different polymer/dye molar ratios (P/D = 0-40). A complete reversal of metachromasy was observed upon addition of co-solvents, suggesting the breakaway of dye molecules from the biopolymer matrix. Binding constant, changes in free energy, enthalpy and entropy of the dye polymer complex were also computed from the spectral data at different temperatures to reveal the nature of the interaction. Quenching of fluorescence of AO by the polymers and the incorporated mechanisms were also explored.
Page(s): 192-197
ISSN: 0301-1208
Source:IJBB Vol.46(2) [April 2009]

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