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|Title:||Optimization of physical parameters for lipase production from <i style="">Arthrobacter</i> sp. BGCC#490|
<i style="">Arthrobacter</i> sp.
Face centered central composite design
Response surface methodology
|Abstract:||The physical parameters for the production of thermostable, alkaline lipase from <i style="">Arthrobacter</i> sp. BGCC# 490 were optimized using response surface methodology (RSM), employing face centered central composite design (FCCCD). The design was employed by selecting pH, temperature and incubation period as the model factors and to achieve maximum yield, interaction of these factors was studied by RSM. A second-order quadratic model and response surface method showed that the optimum conditions for lipase production (pH 10.0, temperature 40<sup>o</sup>C and incubation period 48 h) resulted in 1.6-fold increase in lipase production (13.75 EUml<sup>-1</sup>), as compared to the initial level (8.6 EUml<sup>-1</sup>) after 48 h of incubation, whereas its value predicted by the quadratic model was 12.8 EUml<sup>-1</sup>. Lipase showed stability in the pH range 8-10 and temperature range 40-60<sup>o</sup>C, with maximum activity at pH 9.0 and temperature 50<sup>o</sup>C. Lipase activity was enhanced in the presence of K<sup>+</sup>, Ca<sup>2+</sup> and Mg<sup>2+</sup> ions, but inhibited by Hg<sup>2+</sup> ions. The enzyme<sup> </sup>exhibited high activity in the presence of acetone, isopropanol and ethanol, but was unaffected by methanol. These properties suggest that the lipase may find potential applications in the detergent industry. The present work also demonstrated the feasibility of using experimental design tools to optimize physical parameters for lipase production by an indigenous <i style="">Arthrobacter</i> sp.|
|Appears in Collections:||IJBB Vol.46(2) [April 2009]|
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