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Title: Phytochemical analysis of Bacopa monnieri (L.) Wettst. and their anti-fungal activities
Authors: Jain, Paras
Sharma, HP
Basri, Fauziya
Priya, Kumari
Singh, Pallavi
Keywords: Bacopa monnieri (L.) Wettst.;Antoxidant activity;Phytochemicals estimations;GC-MS analysis
Issue Date: Apr-2017
Publisher: NISCAIR-CSIR, India
IPC Code: Int. Cl.8: A61K 36/00, C09K 15/00, C07, C08, C07C-C07K
Abstract: Realization of hazardous effects of synthetic fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, designed to investigate the anti-fungal activities of Bacopa monnieri (L.) Wettst. and understand its possible mechanism. To estimate the anti-fungal activity, food poisoning method was applied. Extract of Bacopa effectively controlled the growth of chosen fungi as evidenced by the IC50 value - Fusarium oxysporum (31. 25 µg/ml), Sclerotium rolfsii (6.25 µg/ml), Alternaria sp (28.75 µg/ml), Rhizoctonia solani (18.75 µg/ml). Phytochemical screening revealed the presence of Tannin, Phlobatannin, Saponin, Steroid, Flavonoid, Cardiac Glycoside, Phenol, Carbohydrate and Alkaloid. However, Anthraquinone and Terpenoid were absent. Quantitative analysis revealed the presence of 12.5 mg, 110 mg and 1.5 mg of tannin, alkaloid and saponin, respectively per gm dry matter of the plant extract, while phenol was estimated to be 24.75 mg GAE/gm methanolic extract. GC-MS analysis showed the presence of 35 compounds in the methanolic extract of whole plant of Bacopa monnieri. The Bacopa monnieri showed free radicals scavenging activity by DPPH method (94 % inhibition at 1000 µg/ml), CUPRAC assay (0.22622 TE mM at 1000 µg/ml), anthocyanin content (1.436 CGE mg/ml), flavonoid content (29.666 GAE /gm). In, conclusion, the plant extracts of Bacopa monnieri contains phytochemicals, which shows antifungal activities.
Page(s): 310-318
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Appears in Collections:IJTK Vol.16(2) [April 2017]

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