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Title: RP-HPLC quantification of five phenolic compounds in Biophytum sensitivum (L.) DC. (Oxalidaceae) and their biological evaluation
Authors: Kumar, Bhanu
Shukla, Pushpendra Kumar
Niranjan, Abhishek
Misra, Ankita
Rawat, Ajay Kumar Singh
Srivastava, Sharad
Keywords: Biophytum sensitivum;Oxalidaceae;HPLC;Phenolics;Antioxidant;Antidiabetic
Issue Date: Apr-2017
Publisher: NISCAIR-CSIR, India
IPC Code: Int. Cl.8: A61K 36/00, C09K 15/00, A01D 16/02, C07C 39/00
Abstract: Biophytum sensitivum (L.) DC. (Oxalidaceae) is a widely used medicinal plant in traditional medicinal systems across the globe. The present study deals with the RP-HPLC quantification of five phenolic acids, along with the in vitro antioxidant and antidiabetic activity of the aerial parts ofthe plant as a scientific investigation of traditional claims of its use as an antioxidant and antidiabetic agent. Anatomy of the stem was done to aid in exploring identification parameters for this plant. Among the five identified phenolic markers, caffeic acid exhibits the highest concentration (352.47 µg/gm), followed by ferulic acid (242.28 µg/gm), gallic acid (233.55 µg/gm), chlorogenic acid (192.06 µg/gm), and rutin (64.67 µg/gm).
The total phenolic and flavonoid contents in the methanolic extract were found to be 87.0 ± 0.404 mg/gm GAE and
14.268 ± 0.055 mg/gm QE, respectively. The IC50 value for the in vitro DPPH method was 0.164 ± 0.411 mg/ml. In vitro antidiabetic activity was analyzed by the starch-iodine assay and the 3,5-DNS method which displayed IC50 values of 0.636 ± 0.05 mg/ml and 1.214 ± 0.04 mg/ml, respectively. The in vitro study results suggest promising antioxidant and antidiabetic activity of the plant which supports its use in the traditional systems of medicine.
Page(s): 319-324
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Appears in Collections:IJTK Vol.16(2) [April 2017]

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