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Title: Acclimatization of neem microshoots adaptable to semi-sterile conditions
Authors: Lavanya, M
Venkateshwarlu, B
Devi, B Poornasri
Keywords: Bio-control agent
hardening
micropropagation
neem
Trichoderma
Issue Date: Apr-2009
Publisher: CSIR
Abstract: The hardening of in vitro propagated microshoots of neem (Azadirachta indica A. Juss.) was carried out using 3 methods under semi-sterile conditions in low cost mini-polytunnels and a shade house. The percentage survival and rooting response was 16.25% in the first (2:1, v/v, sand and soil with 1′′ × 1′′ central cylindrical cocopeat plugs) and second method (1:1, v/v, cocopeat : bio-fertilizer), but was 100% in the third method (2:1, v/v, sand and soil with 1:1, v/v, cocopeat:biofertilizer and addition of Trichoderma viride. During the acclimatization process, the chlorophyll content in leaves gradually increased from 0.97 (stage I) to 1.35 (stage II), 1.56 (stage III) and 2.14 mg/g (stage IV), indicating a shift in the mode of nutrition from heterotrophic through myxotrophic to autotrophic. Similarly, the percentage water loss from the leaves of plantlets decreased from 90.38 (stage I) to 46.83% (stage IV), indicating stomatal development and progressive hardening. Ex vitro rooting and use of the bio-control agent could bring down the cost of production and make micropropagation of neem feasible and to be adopted as a rural enterprise.
Page(s): 218-222
ISSN: 0972-5849
Source:IJBT Vol.08(2) [April 2009]

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