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Title: Acclimatization of neem microshoots adaptable to semi-sterile conditions
Authors: Lavanya, M
Venkateshwarlu, B
Devi, B Poornasri
Keywords: Bio-control agent
<i style="">Trichoderma</i>
Issue Date: Apr-2009
Publisher: CSIR
Abstract: The hardening of <i>in vitro</i> propagated microshoots of neem <i>(Azadirachta indica</i> A. Juss.) was carried out using 3 methods under semi-sterile conditions in low cost mini-polytunnels and a shade house. The percentage survival and rooting response was 16.25% in the first (2:1, v/v, sand and soil with 1′′ × 1′′ central cylindrical cocopeat plugs) and second method (1:1, v/v, cocopeat : bio-fertilizer), but was 100% in the third method (2:1, v/v, sand and soil with 1:1, v/v, cocopeat:biofertilizer and addition of <i style="">Trichoderma viride.</i> During the acclimatization process, the chlorophyll content in leaves gradually increased from 0.97 (stage I) to 1.35 (stage II), 1.56 (stage III) and 2.14 mg/g (stage IV), indicating a shift in the mode of nutrition from heterotrophic through myxotrophic to autotrophic. Similarly, the percentage water loss from the leaves of plantlets decreased from 90.38 (stage I) to 46.83% (stage IV), indicating stomatal development and progressive hardening. <i>Ex vitro</i> rooting and use of the bio-control agent could bring down the cost of production and make micropropagation of neem feasible and to be adopted as a rural enterprise.
Description: 218-222
ISSN: 0972-5849
Appears in Collections:IJBT Vol.08(2) [April 2009]

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