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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(2) [April 2009] >
| Title: | Kappa-casein gene polymorphism in Indian goats |
| Authors: | Kumar, A
Rout, P K
Mandal, A
Roy, R |
| Keywords: | Goat
κ-casein polymorphism
PCR-RFLP
SDS-PAGE
single strand conformation polymorphism |
| Issue Date: | Apr-2009 |
| Publisher: | CSIR |
| Abstract: | The present work was carried out to analyse the k-casein variants in five Indian goat breeds by SDS-PAGE, PCR-RFLP and SSCP method. A total of 152 unrelated blood samples and 102 milk samples belonging to Barbari, Beetal, Marwari, Surti and Local MP (non-descript) goats were used for analysis. SDS-PAGE exhibited k-casein allele A in all the breeds. Moreover PCR+RFLP analysis also confirmed the presence of AA genotype in all the breeds. PCR-SSCP analysis of k-casein genetic variant showed the k-casein A and B allele in the analysed samples. The k-casein A allele was the dominant variant found in all analyzed breeds with frequencies ranging from 0.70 (Barbari) to 0.8 (Local MP). The variant B was most frequent in Barbari goats as compared to other breeds. |
| Page(s): | 214-217 |
| ISSN: | 0972-5849 |
| Source: | IJBT Vol.08(2) [April 2009]
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