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|Title:||Kappa-casein gene polymorphism in Indian goats|
Rout, P K
|Keywords:||Goat;κ-casein polymorphism;PCR-RFLP;SDS-PAGE;single strand conformation polymorphism|
|Abstract:||The present work was carried out to analyse the k-casein variants in five Indian goat breeds by SDS-PAGE, PCR-RFLP and SSCP method. A total of 152 unrelated blood samples and 102 milk samples belonging to Barbari, Beetal, Marwari, Surti and Local MP (non-descript) goats were used for analysis. SDS-PAGE exhibited k-casein allele A in all the breeds. Moreover PCR+RFLP analysis also confirmed the presence of AA genotype in all the breeds. PCR-SSCP analysis of k-casein genetic variant showed the k-casein A and B allele in the analysed samples. The k-casein A allele was the dominant variant found in all analyzed breeds with frequencies ranging from 0.70 (Barbari) to 0.8 (Local MP). The variant B was most frequent in Barbari goats as compared to other breeds.|
|Appears in Collections:||IJBT Vol.08(2) [April 2009]|
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