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IJBT Vol.08(2) [April 2009] >


Title: Kappa-casein gene polymorphism in Indian goats
Authors: Kumar, A
Rout, P K
Mandal, A
Roy, R
Keywords: Goat
κ-casein polymorphism
PCR-RFLP
SDS-PAGE
single strand conformation polymorphism
Issue Date: Apr-2009
Publisher: CSIR
Abstract: The present work was carried out to analyse the k-casein variants in five Indian goat breeds by SDS-PAGE, PCR-RFLP and SSCP method. A total of 152 unrelated blood samples and 102 milk samples belonging to Barbari, Beetal, Marwari, Surti and Local MP (non-descript) goats were used for analysis. SDS-PAGE exhibited k-casein allele A in all the breeds. Moreover PCR+RFLP analysis also confirmed the presence of AA genotype in all the breeds. PCR-SSCP analysis of k-casein genetic variant showed the k-casein A and B allele in the analysed samples. The k-casein A allele was the dominant variant found in all analyzed breeds with frequencies ranging from 0.70 (Barbari) to 0.8 (Local MP). The variant B was most frequent in Barbari goats as compared to other breeds.
Page(s): 214-217
ISSN: 0972-5849
Source:IJBT Vol.08(2) [April 2009]

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