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|Title: || Micropropagation of Baliospermum montanum (Willd.) Muell. Arg.— A threatened medicinal plant |
|Authors: ||Sasikumar, S|
|Keywords: ||Baliospermum montanum;micropropagation;threatened plant|
|Issue Date: ||Apr-2009|
|Abstract: || An efficient in vitro regeneration protocol was developed for Baliospermum montanum (Willd.) Muell. Arg., a wild threatened medicinal plant. The plants were regenerated from young nodal buds and shoot tips. The morphogenic frequency of shoot bud induction and shoot multiplication was significantly higher in nodal segments when compared to shoot tips. Maximum number of shoots (22.2 ± 0.84) with high frequency of shooting response (82%) was obtained in nodal explants cultured on MS medium fortified with 2.0 mg L-1 BAP. Maximum shoot height of 15.8 cm was achieved. Caulogenic effect of BAP was found to be significant compared to Kn. The excised shoots were cultured on MS medium with various concentrations and combinations of auxins for rooting. Maximum number of healthy rootlets (14.8 ± 2.07 cm) with 90% rooting response was observed due to a synergistic action of IBA (1.0 mg L-1) and IAA (0.5 mg L-1) on half strength MS basal medium. The regenerated plantlets were transferred to the natural habitat with 86.2% success. |
|Appears in Collections:||IJBT Vol.08(2) [April 2009]|
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