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Title: Efficacy of non-purine and purine cytokinins on shoot regeneration in vitro in sugarcane
Authors: Vinayak, Vandana
Dhawan, Ashok K
Gupta, V K
Keywords: 4-CPPU
shoot regeneration
in vitro
growth regulators
Issue Date: Apr-2009
Publisher: CSIR
Abstract: Effect of two non-purine cytokinins, TDZ [N-phenyl-N' - (1,2,3-thidiazol-5-yl) urea] and 4-CPPU [N- (2-chloro-4-pyridyl) N-phenylurea] and meta-topolin [6 (3-hydroxybenzylamino) purine] a biologically active aromatic compound, on shoot regeneration in sugarcane var. CoS 8436 and Co 1148 and S. officinarum clone ‘Gungera’ was observed at 15, 25 and 35 d. 4-CPPU significantly enhanced shoot regeneration in var. CoS 8436, but showed no effect or caused only a slight promotion in var. Co 1148 and clone ‘Gungera’. Meta-topolin was most effective in promoting shoot regeneration at 5 µM in var CoS 8436, at 1 µM in var Co 1148 and at 10 µM in in clone Gungera. The best response to TDZ was observed at 0.01 µM in vars CoS 8436 and Co 1148 and at 0.1 µM in clone Gungera. Thus, there was a clear varietal response of sugarcane to these chemicals. Clone ‘Gungera’ responded best to meta-topolin, CoS 8436 to 4-CPPU and Co 1148 to TDZ. Results obtained in this work clearly demonstrate TDZ induced shoot regeneration in sugarcane at unusually low concentration, such as 0.01 µM and 0.1 µM. Further, 4-CPPU, meta-topolin and TDZ simulate cytokinin activity and may thus provide a better substitute of cytokinins like BAP or Kn that are generally used in tissue cultur
Page(s): 227-231
ISSN: 0972-5849
Source:IJBT Vol.08(2) [April 2009]

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