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Title: Efficacy of non-purine and purine cytokinins on shoot regeneration <i style="">in vitro</i> in sugarcane
Authors: Vinayak, Vandana
Dhawan, Ashok K
Gupta, V K
Keywords: 4-CPPU
shoot regeneration
<i style="">in vitro</i>
growth regulators
Issue Date: Apr-2009
Publisher: CSIR
Abstract: Effect of two non-purine cytokinins, TDZ [N-phenyl-N' - (1,2,3-thidiazol-5-yl) urea] and 4-CPPU [N- (2-chloro-4-pyridyl) N-phenylurea] and meta-topolin [6 (3-hydroxybenzylamino) purine] a biologically active aromatic compound, on shoot regeneration in sugarcane var. CoS 8436 and Co 1148 and <i>S. officinarum</i> clone ‘Gungera’ was observed at 15, 25 and 35 d. 4-CPPU significantly enhanced shoot regeneration in var. CoS 8436, but showed no effect or caused only a slight promotion in var. Co 1148 and clone ‘Gungera’. Meta-topolin was most effective in promoting shoot regeneration at 5 µ<i style="">M</i> in var CoS 8436, at 1 µ<i style="">M</i> in var Co 1148 and at 10 µ<i style="">M</i> in in clone Gungera. The best response to TDZ was observed at 0.01 µ<i style="">M</i> in vars CoS 8436 and Co 1148 and at 0.1 µ<i style="">M</i> in clone Gungera. Thus, there was a clear varietal response of sugarcane to these chemicals. Clone ‘Gungera’ responded best to meta-topolin, CoS 8436 to 4-CPPU and Co 1148 to TDZ. Results obtained in this work clearly demonstrate TDZ induced shoot regeneration in sugarcane at unusually low concentration, such as 0.01 µ<i style="">M</i> and 0.1 µ<i style="">M</i>. Further, 4-CPPU, meta-topolin and TDZ simulate cytokinin activity and may thus provide a better substitute of cytokinins like BAP or Kn that are generally used in tissue cultur
Description: 227-231
ISSN: 0972-5849
Appears in Collections:IJBT Vol.08(2) [April 2009]

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